TABLE 1.
Primers were used in this study.
| Primers | Primer Sequences (5′–3′) |
| cDNA clone | |
| CG12116F | ATGGCAGCAAAGAGAATGGATT |
| CG12116R | GTCATAAAACGAAGGTGTCGTAATAGT |
| SptrF | ATGGACCTGAAACAGCGCAC |
| SptrR | GGAACTGCTCATCCCTGTAATCCA |
| LvSprF | GGCAAGCAAGACTGACAT |
| LvSprR | AACGGAACGAAGACCATC |
| LvSprGSP2 | TGGCTCCAAGTGCGGCAATAGAGGTGATA |
| LvSprNGSP2 | CTGTGTGCAGTGCAGCCCTTCAAGTCAT |
| LvSprGSP1 | CTTGAAGGGCTGCACTGCACACAGAGAC |
| LvSprNGSP1 | ATTGCCGCACTTGGAGCCAGTGATCGTA |
| dsRNA template amplification | |
| dsCG12116-F | GGATCCTAATACGACTCACTATAGGAATCGAAGCACTTGGAGCGA |
| dsCG12116-R | GGATCCTAATACGACTCACTATAGGGATCAGCTTGAGTGTGGCCT |
| dsSptr-F | GGATCCTAATACGACTCACTATAGGCGTGCAAACCTATTCGCTGG |
| dsSptr-R | GGATCCTAATACGACTCACTATAGGAAGTACATCTCACGGGCTGC |
| dsLvSpr-F | GGATCCTAATACGACTCACTATAGGCGAATGTGCCAAGTCCC |
| dsLvSpr-R | GGATCCTAATACGACTCACTATAGGTCAGCAGCCAAGACCTTAA |
| dsEGFP-F | GGATCCTAATACGACTCACTATAGGTCAGCGTGTCCGGCGAG |
| dsEGFP-R | GGATCCTAATACGACTCACTATAGG TCTTCTGCTTGTCGGCC |
| dsWSV056-F | GGATCCTAATACGACTCACTATAGGTTGGCGTATTTCTTCCC |
| dsWSV056-R | GGATCCTAATACGACTCACTATAGGCCACATTGGCATTCATTC |
| Subcellular localization | |
| pEGFP-CG12116-FF | GACTCAGATCTCGAGATGGCAGCAAAGAGAATGGATT |
| pEGFP-CG12116-R | CGACTGCAGAATTCGTCATAAAACGAAGGTGTCGTAATAGT |
| pEGFP-Sptr-F | GACTCAGATCTCGAGATGGACCTGAAACAGCGCAC |
| pEGFP-Sptr-R | CGACTGCAGAATTCGGAACTGCTCATCCCTGTAATCCA |
| Dual-luciferase reporter assay | |
| pCMV-HA-WSV056-F | CGGAATTCGGATGGCCTCAGTCTTTGAAGA |
| pCMV-HA-WSV056-R | GGGGTACCTTATTGTACCAAAAACTCAGAAATC |
| pGL3-CG12116P-F | ATAGGTACCGAGCTCCAAAAGGGGCTTGAAAAAATTAG |
| pGL3-CG12116P-R | CGGAATGCCAAGCTTTATTTTGATTTAAAGGATTTCGGGT |
| pGL3-SptrP-F | ATAGGTACCGAGCTCTTTCGCATCGAGTGAAGCTTAA |
| pGL3-SptrP-R | CGGAATGCCAAGCTTGATTGCGTGAAAAACACGGAC |
| Real-time quantitative PCR | |
| Rp49-qF |
ATGACCATCCGCCCAGCATAC |
| Rp49-qR | ATGACCATCCGCCCAGCATAC |
| CG12116-qF | TTCAGTTCCCGAAATCCTCAA |
| CG12116-qR | CAGGGATTGACCCAAAGGATT |
| LvSpr-qF | TACGGTGTTGAACTATGCTCC |
| LvSpr-qR | AACAGATGTGCTACACGGAAG |
| Sptr-qF | ATCAAAGCCGAGGGTTCCAT |
| Sptr-qR | ATCAAAGCCGAGGGTTCCAT |
| WSV056-qF | GGCAAAGTTCCTACCCG |
| WSV056-qR | TGCTTCAAATGCTCCAC |
| WSSV-qF | AGTTGGCACCTTTGTGTGTGGTA qWSSV-R TTTCCACCGGCGGTAGCT |
| WSSV-qR | TTTCCACCGGCGGTAGCT |
| Lvβ-actin-qF | AGGCTAACCGCGAGAAGATGAC |
| Lvβ-actin-qR | GTAGCACAGTTTCTCCTTGATG |