TABLE 3.
Powdery mildew infected leaf area rates of representative edited and non-edited grapevine plants under greenhouse conditions.
| Target gene | Genotype | Line number | Infected leaf area (%)* | Reduction of infected leaf area (%)** | |
| VviDEL1 | Aa | 553-1 | 0,0 | * | 100,0 |
| VviLIN2 | Aa | 378-3 | 3,4 | ns | 41,6 |
| VviDEL1 | Aa | 453 | 3,4 | ns | 40,6 |
| VviSWEET4 | AA | 393-2 | 4,5 | ns | 21,7 |
| VviSWEET4 | AA | 393-1 | 4,7 | ns | 18,7 |
| VviDEL1 | AA | 558-2 | 5,1 | ns | 12,3 |
| WT | 5,8 | 0,0 | |||
| VviLIN2 | Aa | 378-2 | 6,5 | ns | –13,2 |
| VviDEL1 | Aa | 432 | 7,3 | ns | –25,6 |
| VviLIN2 | Aa | 378-1 | 7,5 | ns | –29,5 |
*Infected leaf area corresponds to affected area mean by powdery mildew compared to total area of three leaves per plant. Image analysis was performed using ImageJ 1.49v software.
**Reduction of infected leaf area corresponds to decrease of the tissue affected by E. necator in percentage compared to WT. Negative values indicate that the symptoms/signs developed were greater than in WT. Values with asterisks indicate significant differences compared with the WT according to Fisher’s LSD test (p < 0.05).
ns, non-significant. AA, non-edited; Aa, Heterozygous/Monoallelic.
Bold values obtained for wild type plants.