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. 2021 Dec 23;12:791030. doi: 10.3389/fpls.2021.791030

TABLE 3.

Powdery mildew infected leaf area rates of representative edited and non-edited grapevine plants under greenhouse conditions.

Target gene Genotype Line number Infected leaf area (%)* Reduction of infected leaf area (%)**
VviDEL1 Aa 553-1 0,0 * 100,0
VviLIN2 Aa 378-3 3,4 ns 41,6
VviDEL1 Aa 453 3,4 ns 40,6
VviSWEET4 AA 393-2 4,5 ns 21,7
VviSWEET4 AA 393-1 4,7 ns 18,7
VviDEL1 AA 558-2 5,1 ns 12,3
WT 5,8 0,0
VviLIN2 Aa 378-2 6,5 ns –13,2
VviDEL1 Aa 432 7,3 ns –25,6
VviLIN2 Aa 378-1 7,5 ns –29,5

*Infected leaf area corresponds to affected area mean by powdery mildew compared to total area of three leaves per plant. Image analysis was performed using ImageJ 1.49v software.

**Reduction of infected leaf area corresponds to decrease of the tissue affected by E. necator in percentage compared to WT. Negative values indicate that the symptoms/signs developed were greater than in WT. Values with asterisks indicate significant differences compared with the WT according to Fisher’s LSD test (p < 0.05).

ns, non-significant. AA, non-edited; Aa, Heterozygous/Monoallelic.

Bold values obtained for wild type plants.