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. 2021 Dec 23;12:795730. doi: 10.3389/fmicb.2021.795730

FIGURE 2.

FIGURE 2

Regulation of pUL48 on promoters of different lengths of intermediate-early (IE) gene. The regulation of pUL48 on ICP4/ICP22/ICP27 promoters of different lengths. ICP4-Pro (740 bp)-Luc, ICP4-Pro (1,235 bp)-Luc, ICP22-Pro (900 bp)-Luc, ICP4-Pro (1,200 bp)-Luc, ICP4-Pro (1,500 bp)-Luc, ICP27-Pro (100 bp)-Luc, and ICP27-Pro (1,500 bp)-Luc were cotransfected into DEF with pCAGGS and pCAGGS-pUL48-HA and pRL-TK, respectively. The transfection ratio of pCAGGS and pCAGGS-pUL48-HA with each double luciferase plasmid was 1:1, and the transfection quantity of pRL-TK was 1/20 of each double luciferase plasmid. Cell samples were collected 24 h after transfection. The activity of each promoter was detected by the dual-luciferase reporting system. pCAGGS-pUL48-HA was transfected into DEF. At 24 h after transfection, cellular protein samples were collected for Western blot analysis. Student’s t-test was used to analyze the differences of the two groups. The significance was as follows: *p < 0.05, ***p < 0.001, ****p < 0.0001.