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. 2021 Dec 23;12:795730. doi: 10.3389/fmicb.2021.795730

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Copyright © 2021 Zhou, Fan, Wang, Cheng, Wu, Yang, Tian, Jia, Ou, Mao, Sun, Zhang, Zhu, Chen, Liu, Zhao, Huang, Gao, Yu and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The regulatory effects of pUL48 on ICP4 and ICP22 promoters containing different components. (A) Initial upstream cis-element sequence analysis of ICP4 and ICP22. Different colored squares represent different possible sequences of cis-elements. (B) The regulation of pUL48 on ICP4 promoter containing different elements. (C) The regulation of pUL48 on ICP22 promoter containing different elements. ICP4-Pro (740 bp)-Luc, ICP4-Pro (900 bp)-Luc, ICP4-Pro (1,226 bp)-Luc, ICP4-Pro (1,235 bp)-Luc, ICP22-Pro (300 bp)-Luc, ICP22-Pro (400 bp)-Luc, ICP22-Pro (900 bp)-Luc, pCAGGS, pCAGGS-pUL48-HA, and pRL-TK were cotransfected into DEF. At 24 h after transfection, cell samples were collected, and the activity of each promoter was detected by the dual-luciferase reporting system. pCAGGS-pUL48-HA was transfected into DEF. At 24 h after transfection, cell protein samples were collected for Western blot analysis. Student’s t-test was used to analyze the differences of the two groups. NS was not significant, ^****p < 0.0001.