FIGURE 5.

Identification of the core region of the DPV pUL48 transcriptional activation domain. The regulation of ICP4/ICP22/ICP27 promoter by a deletion in different regions of the pUL48 transcriptional activation domain. The N-terminal-truncated plasmids of pUL48 were cotransfected into DEF with ICP4-Pro (1,235 bp), ICP22-Pro (900 bp), ICP27-Pro (1,500 bp), and pRL-TK. pCAGGS was the negative control, and pCAGGS-pUL48-HA was the positive control. Cell samples were collected 24 h after transfection, and the activity of each promoter was detected by a dual-luciferase reporting system. The pUL48 N-terminal-truncated plasmid was transfected into DEF. At 24 h after transfection, cellular protein samples were collected for Western blot analysis. Student’s t-test was used to analyze the differences of the two groups, and the significance was as follows: ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001.