TABLE 1.
Design of luciferase reporter plasmids for different gene promoters.
| The purpose gene | Primer | 5′–3′ sequences |
| ICP4-Pro (900 bp) | ICP4-Pro-1 F | CTGGCCTAACTGGCCGGTACC ATATGGTTTAATTATAT |
| ICP4-Pro-1 R | AGCTTGGCCGCCGAGGCCAGATCT GAGTAGAGCTGGGCGTCTATTA |
|
| ICP4-Pro (1,226 bp) | ICP4-Pro-2 F | CTGGCCTAACTGGCCGGTACC GCACTGCAGGATGTTTTA |
| ICP4-Pro-2 R | ICP4-Pro-1 R | |
| ICP4-Pro (1,235 bp) | ICP4-Pro-3 F | TCTCTGGCCTAACTGGCCGGTACC TAATGATATGCACTGCAGGATG |
| ICP4-Pro-3 R | ICP4-Pro-1 R | |
| ICP22-Pro (300 bp) | ICP22-Pro-1 F | TCTCTGGCCTAACTGGCCGGTACC TTGCTCAATACGGGA |
| ICP22-Pro-1 R | AGCTTGGCCGCCGAGGCCAGATCT GTCTATCGCAGTTCT | |
| ICP22-Pro (400 bp) | ICP22-Pro-2 F | TCTCTGGCCTAACTGGCCGGTACC |
| ICP22-Pro-2 R | ICP22-Pro-1 R | |
| ICP22-Pro (900 bp) | ICP22-Pro-3 F | TCTCTGGCCTAACTGGCCGGTACC CTCTAATGCATATTC |
| ICP22-Pro-3 R | ICP22-Pro-1 R | |
| ICP22-Pro (1,114 bp) | ICP22-Pro-4 F | TCTCTGGCCTAACTGGCCGGTACC CAGCGGTGCAGCCGT |
| ICP22-Pro-4 R | ICP22-Pro-1 R | |
| ICP22-Pro (1,200 bp) | ICP22-Pro-4 F | TCTCTGGCCTAACTGGCCGGTACC CCCCATCCCGATAGT |
| ICP22-Pro-5 R | ICP22-Pro-1 R | |
| ICP22-Pro (1,500 bp) | ICP22-Pro-6 F | TCTCTGGCCTAACTGGCCGGTACC AATCCAAAATGCCAG |
| ICP22-Pro-6 R | ICP22-Pro-1 R | |
| ICP27-Pro (1,000 bp) | ICP27-Pro-1 F | CTGGCCTAACTGGCCGGTACC TCACCTAATGGGGCCA |
| ICP27-Pro-1 R | AGCTTGGCCGCCGAGGCCAGATCT GACGTTGGGCAGGTTCGTTA | |
| ICP27-Pro (1,500 bp) | ICP27-Pro-2 F | CTGGCCTAACTGGCCGGTACC CATGAGTATAAGAATGCGATCGC |
| ICP27-Pro-2 R | ICP27-Pro-1 R | |
| UL28-Pro (495 bp) | UL28-Pro F | TCTCTGGCCTAACTGGCCGGTACC TGGGGCAACGCGTACAAT |
| UL28-Pro R | AGCTTGGCCGCCGAGGCCAGATCT TTTCCAGATAGTGTTTG |
|
| TK-Pro (555 bp) | TK-Pro F | TCTCTGGCCTAACTGGCCGGTACC GCTGGGCTACAAATA |
| TK-Pro R | AGCTTGGCCGCCGAGGCCAGATCT TTGATCGGAAATGTA |
|
| UL48-Pro (349 bp) | UL48-Pro F | TCTCTGGCCTAACTGGCCGGTACC TCTGGCCAGACCCCTCGT |
| UL48-Pro F | AGCTTGGCCGCCGAGGCCAGATCT ATCCACACCCACACCTAT |