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. Author manuscript; available in PMC: 2022 Apr 28.
Published in final edited form as: Nat Methods. 2021 Oct 28;18(11):1377–1385. doi: 10.1038/s41592-021-01303-3

Extended Data Fig. 5. Evidence for the additional thiamine-derived metabolites.

Extended Data Fig. 5

Evidence for the additional thiamine-derived metabolites. Similar to Figure 3, adding unlabeled thiamine to [U-13C]glucose culture media, yeast uptake the unlabeled thiamine, resulting in unlabeled thiamine, M+4 labeled thiamine+[C4H6O3] and thiamine+[C4H8O] species (n=5). The proposed formulae are also supported by m/z measured by high-resolution mass-spectrometry. Bar represents mean values and error bar indicates s.d..