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. 2021 Dec 23;12:767726. doi: 10.3389/fimmu.2021.767726

Figure 5.

Figure 5

Allelic differential expression of susceptible gene mediated by SCV2-miRNAs. (A) Flowchart of the screening process of interaction between SCV2-miRNAs and susceptible polymorphisms sites associated with COVID-19. (B) Graphic table representing the information that COVID-19-susceptible polymorphisms significantly altered SCV2-miR-ORF1ab-2-5p interaction with its target. (C) The schematic diagram displays the energy change between the two alleles of rs7967461 (G→C) interacting with SCV2-miR-ORF1ab-5p. The value of energy change is depicted above the dotted black horizontal lines. The red arrow indicates the base alteration from Ref-type to the Alt one. (D) Variation in the OAS1 3′UTR differentially affects the reporter gene’s expression. The sequences containing OAS1-rs7967461 single-nucleotide polymorphism (SNP) allele (78 bp) were cloned into psiCHEK2 reporter plasmid. HEK293T cells were co-electroporated with SCV2-miR-ORF1ab-2 and SNP reporter plasmid. Forty-eight hours later, luciferase activity in cell lysates was detected. Data are expressed as mean ± SEM of three independent experiments. p-Values were analyzed with two-tailed unpaired t-test. **p < 0.01.