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. 2022 Jan 6;41:11. doi: 10.1186/s13046-021-02220-1

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — AHSA1 promotes MM proliferation and BTZ resistance through activating CDK6 and PSMD2 respectively. A-C Using Co-IP assay followed by MS, CDK6 and PSMD2 were selected among candidate genes of the proliferation-related and drug-resistance genes in ARP1 WT, AHSA1-OE cells, AHSA1-OE cells treated with Bufalin or BTZ, respectively. D WB analysis showed the expressions of CDK6 and PSMD2 in AHSA1-OE (Left) and AHSA1-KD cells (Right). E Co-IP experiment further confirmed the interaction between HSP90 with CDK6 and PSMD2 in ARP1 and H929 cells using HSP90 antibody as bait. F Co-IP experiment showed that CDK6 directly interacted with HSP90 in ARP1 and H929 cells. G Co-IP assay confirmed that PSMD2 interacted with HSP90 in ARP1 and H929 cells. H Proteasome activity assay showed that overexpression of AHSA1 in ARP1 and H929 cells resulting in high proteasome activity. I Validation of PSMD2 overexpression in ARP1 and H929 PSMD2-OE cells relative to WT cells. J Proteasome activity assay showed that overexpression of PSMD2 in ARP1 and H929 cells led to high proteasome activity. K-L Effects of BTZ on the cell viability of ARP1 (K) and H929 (L) cells with or without PSMD2 overexpression. The data are expressed as mean ± SD.*p<0.05, **p<0.01, ***p<0.001