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Identification of Class I histone deacetylase enzymes HDAC1 and HDAC3 in mouse sperm. Expression of HDAC1 and HDAC3 was assayed in immature and mature sperm using Western blotting. We derived protein extracts from 7.5 million caput spermatozoa and 38 million cauda sperm. Note: we cannot quantitatively compare bands between immature and mature sperm as we loaded unequal amounts of protein
