Table 3.

Advantages and Disadvantages of enzymatic decellularization

Technique	Mechanism	Advantages	Disadvantages	Reference
Enzymatic Decellularization	Proteases: trypsin: Breaks down cellular proteins on the c-side of Arg or Lys and then destroys ECM proteins such as collagen and elastin. Pepsine: Breaks the bounds between peptides Nucleases: Break sequences of nucleic acids.	highly effective	Can damage the proteins in the ECM, especially laminin and GAG It changes the structure of the matrix Further cleaning and enzyme removal is required They may promote immune response.	[75]