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. 2022 Jan 3;10:e12263. doi: 10.7717/peerj.12263

Figure 3. miR-100-5p targets and reduces CDC25A level in BC cells.

Figure 3

(A) Venn diagram of up-regulated DEmRNAs and predicted downstream genes of miR-100-5p; (B) Pearson correlation analysis of miR-100-5p and predicted genes CDC25A, FGFR3 and PCSK9; (C) Pearson correlation analysis of miR-100-5p and CDC25A; (D) box plots of CDC25A level (green refers to normal and red refers to tumor; normal sample: 113; tumor sample: 1109); (E) Western blot assessed CDC25A expression in MCF-10A, T47D, MDA-MB-231, MCF-7, and BT-474; (F) the binding sequences of miR-100-5p and CDC25A; (G) binding of miR-100-5p and CDC25A validated through dual-luciferase assay; (H–I) CDC25A mRNA and protein levels in MCF-7 cells; * P < 0.05; Experiments in Figure E, G–I was repeated three times, including three technical replicates and three biological replicates, and representative figures were selected.