TABLE 1.
B. cepacia complex strains used to develop molecular diagnostic approaches
| Genomovar and straina | 16S rDNA DdeI RFLPb | Result of recA RFLP by digestion with:
|
recA GenBank accession no. | recA phylogenetic and PCR identification group | |
|---|---|---|---|---|---|
| HaeIIIc | MnlId | ||||
| B. cepacia genomovar I | |||||
| ATCC 25416T | 2 | D | d | AF143786 | |
| J1050e | 2 | D | d | I | |
| ATCC 17759 | 2 | E | e | AF143788 | I |
| CEP509 | 2 | E | e | AF143787 | I |
| LMG 17997 | 2 | E | e | I | |
| B. multivorans (formerly genomovar II) | |||||
| C5393 | 3 | F | a | AF143776 | B. multivorans |
| LMG 13010T | 3 | F | a | B. multivorans | |
| CF-A1-1 | 3 | F | a | B. multivorans | |
| C1576 | 3 | C | a | AF143774 | B. multivorans |
| HI-2308e | 3 | C | a | AF143777 | B. multivorans |
| JTC | 3 | F | a | AF143778 | B. multivorans |
| C1962 | 3 | F | a | B. multivorans | |
| ATCC 17616 | 3 | F | a | AF143775 | B. multivorans |
| 249-2 | 3 | F | a | B. multivorans | |
| B. cepacia genomovar III | |||||
| J2315 | 2 | G | f | III-A | |
| BC7 | 2 | G | f | III-A | |
| K56-2 | 2 | G | f | AF143779 | III-A |
| C5424 | 2 | G | f | AF143781 | III-A |
| C6433 | 2 | G | f | AF143780 | III-A |
| C4455e | 2 | G | f | AF143782 | III-A |
| C1394 | 2 | H | g | AF143783 | III-B |
| ATCC 17765 | 2 | H | g | III-B | |
| CEP511 | 2 | I | h | AF143785 | III-B |
| PC184 | 2 | J | i | AF143784 | III-B |
| B. stabilis (formerly genomovar IV) | |||||
| LMG 07000e | 2 | J | b | AF143789 | B. stabilis |
| LMG 14291e | 2 | J | b | AF143790 | B. stabilis |
| LMG 14294 | 2 | J | b | B. stabilis | |
| C7322 | 2 | J | b | B. stabilis | |
| LMG 14086 | 2 | J | b | B. stabilis | |
| LMG 18888 | 2 | J | b | B. stabilis | |
| B. vietnamiensis (formerly genomovar V) | |||||
| PC259 | 1 | A | c | AF143791 | B. vietnamiensis |
| LMG 16232 | 1 | A | c | B. vietnamiensis | |
| FC441 | 1 | A | c | B. vietnamiensis | |
| LMG 10929T | 1 | B | c | AF143793 | B. vietnamiensis |
| C2822e | 1 | B | c | AF143792 | B. vietnamiensis |
All strains except those indicated in footnote b below were derived from the B. cepacia complex strain panel where source and other data relevant to each are described (18).
Data are numerical 16S rRNA RFLP types as indicated in Fig. 1.
Letters correspond to alphabetical RFLP types as shown in Fig. 2A.
Letters correspond to alphabetical RFLP types as shown in Fig. 2B.
Strains not derived from the B. cepacia complex panel (18): J1050, isolated from non-CF patient within the U.K. and kindly provided by J. R. W. Govan; HI-2308, recovered from neonatal infection and kindly provided by J. J. LiPuma; C4455, recovered from a patient with CF (16); LMG 07000, recovered from a patient with septicaemia (20); LMG 14291, recovered from a patient with CF (20); and C2822, recovered from a patient with CF (16).