TABLE 3.
Evaluation of genomovar-specific primers on a collection of genetically diverse B. cepacia complex strains
PCR primer set specificity | No. of isolates testing positive with each primer set | recA HaeIII RFLP type within positive isolates | No. of isolates within each RFLP type | No. of genetically heterogeneous strains within each RFLP type | 16s rDNA RFLP type (DdeI) |
---|---|---|---|---|---|
Genomovar I | 8 | D | 5 | 3 | 2 |
E | 3 | 3 | 2 | ||
B. multivorans | 20 | C | 3 | 3 | 3 |
F | 17 | 16 | 3 | ||
Genomovar III-A | 16 | G | 16 | 8 | 2 |
Genomovar III-B | 24 | H | 4 | 3 | 2 |
I | 10 | 8 | 2 | ||
J | 10 | 7 | 2 | ||
B. stabilis | 22 | J | 22 | 4 | 2 |
B. vietnamiensis | 13 | A | 6 | 5 | 1 |
B | 7 | 6 | 1 | ||
Total | 103 | 10 | 103 | 66 |