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. 2021 Nov 8;33(Suppl 1):e922–e932. doi: 10.1097/MEG.0000000000002309

Fig. 5.

Fig. 5.

KIF9-AS1 directly interacted with miR-148a-3p, and miR-148a-3p directly interacted with suppressor of cytokine signaling (SOCS3). (a) The putative binding site of miR-148a-3p in KIF9-AS1 was predicted by starBase; (b) relative luciferase activity in HT-29 cells was measured by dual-luciferase reporter assay. **P < 0.01 vs. miR-NC; (c) the expression of miR-148a-3p in HT-29 cells was detected by quantitative real time PCR (qRT-PCR). **P < 0.01 vs. si-NC. (d) The binding site for miR-148a-3p on the 3′ UTR of SOCS3 was predicted by targetScan. (e) Dual-luciferase reporter assay was performed to detect the relative luciferase activity in HT-29 cells. **P < 0.01 vs. miR-NC; (e) The protein expression of SOCS3 in HT-29 cells was measured by western blot. **P < 0.01 vs. miR-NC.