Table 4b.

Ratio estimation of dilution series of artificial mixtures

Artificial Mixture	HCMV-DNA	Aspired Input ratio	Estimated copies/ml	Copy input for PCR	Measured ratio2
1	Merlin	1	1,90E+ 05 1	1.90E+ 03	1.5
	TB40-BAC4-luc	99	3,90E+ 06 1	3.90E+ 04	98.5
1:10	Merlin	1	1.90E+ 04	1.90E+ 02	1
	TB40-BAC4-luc	99	3.90E+ 05	3.90E+ 03	99
1:100	Merlin	1	1.90E+ 03	1.90E+ 01	1.5
	TB40-BAC4-luc	99	3.90E+ 04	3.90E+ 02	98.5
1:1000	Merlin	1	1.90E+ 02	1.90E+ 00	0
	TB40-BAC4-luc	99	3.90E+ 03	3.90E+ 01	100

1) original mixture was confirmed by HCMV-specific qPCR (see Table 4a); 2) measured ratios are means of reads mapping to genotypic regions within gN, gO, and gH