Table 2.
MALDI-TOF MS identification of neuropeptides in SLB cells/VWCs of nudipleurans.
| Species | Prohormone family homology | Peptide sequence | Peptide name | Mean M + H | Theoretical M + H | Mass error ppm |
|---|---|---|---|---|---|---|
| H. crassicornis | FCAP | GLDSLGGFNVHGGW | FCAP3 | 1415.684 | 1415.668 | 11.3 |
| FCAP | GLDSLGGFQVHGGW | FCAP1 | 1429.71 | 1429.684 | 18.2 | |
| FCAP | GLDSLGGFHVHGGGW | FCAP2 | 1495.7 | 1495.706 | -4 | |
| FMRFa | FMRFamide | FMRFa | 599.279 | 599.312 | −55.1 | |
| LFRFa | TLFRFamide | TLFRFa | 682.393 | 682.403 | −14.7 | |
| LFRFa | GSIFRFamide | GSIFRFa | 725.404 | 725.409 | −6.9 | |
| LFRFa | ASLFRFamide | ASLFRFa | 739.423 | 739.425 | −2.7 | |
| LFRFa | GGSLFRFamide | GGSLFRFa | 782.464 | 782.431 | 42.2 | |
| LFRFa | ARGSLFRFamide | ARGSLFRFa | 952.594 | 952.547 | 49.3 | |
| SCP | SGYLAFPRMamide | SCPA | 1041.588 | 1041.535 | 50.9 | |
| SCP | VNYLAFPRMamide | SCPB | 1109.638 | 1109.592 | 41.5 | |
| M. leonina | FMRFa | FVRFamide | FVRFa | 567.313 | 567.34 | −47.6 |
| FMRFa | FLRFamide | FLRFa | 581.338 | 581.356 | −31 | |
| FMRFa | YLRFamide | YLRFa | 597.335 | 597.35 | −25.1 | |
| FMRFa | FMRFaide | FMRFa | 599.303 | 599.312 | −15 | |
| FMRFa | RSVDDDDMSTRSGDVID | FMRFa peptides-2 | 1882.809 | 1882.806 | 1.6 | |
| FMRFa | SQQPNVDDIYNKALLQLEEPYS | FMRFa peptides-1 | 2564.237 | 2564.249 | −4.7 | |
| LFRFa | SSLFRFamide | SSLFLRa | 755.442 | 755.42 | 29.1 | |
| LFRFa | GGTLFRFamide | GGTLFRFa | 796.477 | 796.446 | 38.9 | |
| LFRFa | acSSLFRFamide | SSLFRFa (acetylated) | 797.432 | 797.43 | 2.5 | |
| LFRFa | acSGPQSNEGM | LFRF peptides-2 | 948.51 | 948.371 | 146.6 | |
| MIP-related | GPPRFVamide | GPPRFVa | 671.414 | 671.398 | 23.8 | |
| MIP-related | pQAPRFVamide | QAPRFVa (pyroglutamated) | 699.411 | 699.393 | 25.7 | |
| MIP-related | QAPRFVamide | QAPRFVa | 716.442 | 716.42 | 30.7 | |
| MIP-related | YVPRFVamide | YVPRFVa | 779.493 | 779.456 | 47.5 | |
| MIP-related | YIPRFVamide | YIPRFVa | 793.511 | 793.472 | 49.2 | |
| MIP-related | AIQPRFVamide | AIQPRFVa | 829.546 | 829.504 | 50.6 | |
| MIP-related | YDPPRFVamide | YDPPRFVa | 892.514 | 892.4673 | 52.3 | |
| MIP-related | ARSPPRFVamide | ARSPPRFVa | 928.594 | 928.54726 | 50.3 | |
| MIP-related | acGPSLQASEE | MIP-related peptides 1 | 959.519 | 959.43 | 92.8 | |
| MIP-related | YGRPIIPGQIamide | YGRPIIPGQIa | 1112.707 | 1112.6572 | 44.8 | |
| MIP-related | DYDTIFDLLHNSA | MIP-related peptides 2 | 1523.721 | 1523.699 | 14.4 | |
| SCP | acSEFSVSEDamide | SCP peptides-1 | 940.5413 | 940.389 | 162 | |
| SCP | SGYAGFPRMS | SCPC | 1072.541 | 1072.486 | 51.3 | |
| SCP | SNYLAFPRMamide | SCPA | 1097.605 | 1097.556 | 44.6 | |
| SCP | MNYLAFPRMamide | SCPB | 1141.616 | 1141.564 | 45.6 | |
| P. californica | SCP | SGYLAFPRMamide | SCPA | 1041.6403 | 1041.535 | 101.1 |
| SCP | MNYLAFPRMamide | SCPB | 1141.7383 | 1141.564 | 152.7 | |
| FMRFa | ASAGGQRSEESLLREALMQAEEPLY | AEEPLY | Previously characterized | |||
| FMRFa | SEESLLREALMQAEEPLY | AEEPLY’ | ||||
| FMRFa | FLRFamide | FLRFa | ||||
| FMRFa | FMRFamide | FMRFa | ||||
| FMRFa | DVGGGSAAGDAEEEDIISRQILGLGGGQVGESGDVIDGF | FMRFa peptide 3 | ||||
| FMRFa | PSNAALEGLEGE | FMRFa peptide 5 | ||||
| QNFLa | (p-)QLDSIGAGMVSGLHQNFL(Amide) | QNFLa-peptide 5 | ||||
| QNFLa | FDSISSGRLNGFNANFL(Amide) | QNFLa-peptide 6 | ||||
Six SLB cells analyzed from H. crassicornis, four SLB cells from M. leonina, and five VWCs from P. californica. PPM – parts per million. Both mean and theoretical M + H values are the average molecular weight.