|
Reagent type (species) or resource
|
Designation
|
Source or reference
|
Identifiers
|
Additional information
|
| Cell line (Mus musculus) |
Fh1fl/fl
|
Frezza et al., 2011
|
Fh1fl/fl
|
Kidney epithelial cell line |
| Cell line (Mus musculus) |
Fh1-/-CL1
|
Frezza et al., 2011
|
Fh1-/-CL1
|
Kidney epithelial cell line |
| Cell line (Mus musculus) |
Fh1-/-CL19
|
Frezza et al., 2011
|
Fh1-/-CL19
|
Kidney epithelial cell line |
| Cell line (Mus musculus) |
Sdhbfl/fl
|
Cardaci et al., 2015
|
Sdhbfl/fl
|
Kidney epithelial cell line |
| Cell line (Mus musculus) |
Sdhb-/-CL5
|
Cardaci et al., 2015
|
Sdhb-/-CL5
|
Kidney epithelial cell line |
| Cell line (Mus musculus) |
Sdhb-/-CL7
|
Cardaci et al., 2015
|
Sdhb-/-CL7
|
Kidney epithelial cell line |
| Chemical compound, drug |
Dimethyl sulfoxide (DMSO) |
Sigma Aldrich |
D8418 |
|
| Chemical compound, drug |
Fumarate hydratase inhibitor 1 (FHIN-1) |
MedChemExpress |
Cat. No.: HY-100004 |
|
| Chemical compound, drug |
Atpenin A5 (AA5) |
Abcam |
CAS Number: 119509-24-9 |
|
| Chemical compound, drug |
Thenoyltrifluoroacetone (TTFA) |
Sigma Aldrich |
T27006 |
|
| Chemical compound, drug |
L-Buthionine-sulfoximine (BSO) |
Sigma Aldrich |
B2515 |
|
| Chemical compound, drug |
ethylGSH (eGSH) |
Sigma Aldrich |
G1404 |
|
| Chemical compound, drug |
Oligomycin A |
Sigma Aldrich |
75,351 |
|
| Chemical compound, drug |
Puromycin dihydrochloride |
Sigma Aldrich |
P9620 |
|
| Chemical compound, drug |
Antimycin A |
Sigma Aldrich |
A8674 |
|
| Chemical compound, drug |
Carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (FCCP) |
Sigma Aldrich |
C2920 |
|
| Chemical compound, drug |
Rotenone |
Sigma Aldrich |
R8875 |
|
| Chemical compound, drug |
Integrated stress response inhibitor (ISRIB) |
Sigma Aldrich |
SML0843 |
|
| Chemical compound, drug |
Glucose |
Sigma Aldrich |
G8270 |
|
| Chemical compound, drug |
Glutamine |
Sigma Aldrich |
G7513 |
|
| Chemical compound, drug |
Pyruvate |
Sigma Aldrich |
P2256 |
|
| Chemical compound, drug |
U-13C-Glutamine |
Cambridge Isotopes |
CLM-1822-H-PK |
|
| Chemical compound, drug |
35S-methionine |
PerkinElmer |
NEG009L005MC |
|
| Chemical compound, drug |
mitoCDNB |
Booty et al., 2019
|
SML2573* |
*Available on Sigma |
| Antibody |
puromycin antibody clone 12D10 “(mouse monoclonal)” |
Sigma Aldrich |
MABE343 |
“(1:10,000)” |
| Antibody |
anti-Atf4 (D4B8) “(rabbit monoclonal)” |
Cell Signalling Technology |
Rabbit mAb #11,815 |
“(1:800)” |
| Antibody |
anti-p-eIF2α (Ser51) (D9G8) “(rabbit monoclonal)” |
Cell Signalling Technology |
XP Rabbit mAb #3,398 |
“(1:1000)” |
| Antibody |
anti-β-Actin (13E5) “(rabbit monoclonal)” |
Cell Signalling Technology |
Rabbit mAb #4,970 |
“(1:1000)” |
| Chemical compound, drug |
U-13C-Glutamine |
Cambridge Isotopes |
CLM-1822-H-PK |
|
| Chemical compound, drug |
35S-methionine |
PerkinElmer |
NEG009L005MC |
|
| Transfected construct (Mus musculus) |
Silencer select Non-targeting control (NTC) siRNA |
Thermo Scientific |
Catalog # 4390843
|
|
| Transfected construct (Mus musculus) |
Silencer select Atf4 siRNA #1 |
Thermo Scientific |
Catalog # 4390771 Assay ID s62691 |
|
| Transfected construct (Mus musculus) |
Silencer selectAtf4 siRNA #2 |
Thermo Scientific |
Catalog # 4390771 Assay ID s62690 |
|
| Transfected construct (Mus musculus) |
Lipofectamine RNAiMAX |
Thermo Scientific |
Catalog # 13778075 |
|
