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. 2022 Jan 6;13:32–45. doi: 10.18632/oncotarget.28168

Figure 3. SHMT2 silencing inhibits MYCN mRNA and N-Myc protein expression at 24-hours.

Figure 3

The effects of SHMT2 silencing and overexpression in BE(2)-C cells were examined 24 hours after plating to control for differences in proliferation rate and cell-cycle. (A) SHMT2 silencing decreased AKT-2 mRNA expression by 2.0-fold and decreased MYCN mRNA expression by 500-fold. SHMT2 overexpression increased AKT-2 mRNA expression by 1.5-fold and MYCN mRNA expression by 1.2-fold. (B) SHMT2 silencing completely inhibited N-Myc protein expression and SHMT2 overexpression increased N-Myc protein expression 2.3-fold, based on densitometry analysis, reported as a ratio of each protein band density relative to the density of each β-actin control band (protein density: β-actin density).