Table 1.
CDC 2019-novel coronavirus (2019-nCoV) real-time RT-PCR diagnostic Panel: primers and probes sequence
| Description | Oligonucleotide sequence | Label1 |
|---|---|---|
| N1 forward primer | GAC CCC AAA ATC AGC GAA AT | None |
| N1 reverse primer | TCT GGT TAC TGC CAG TTG AAT CTG | None |
| N1 probe | FAM-ACC CCG CAT TAC GTT TGG TGG ACC-BHQ1 | FAM, BHQ-1 |
| N2 forward primer | TTA CAA ACA TTG GCC GCA AA | None |
| N2 reverse primer | GCG CGA CAT TCC GAA GAA | None |
| N2 probe | FAM-ACA ATT TGC CCC CAG CGC TTC AG-BHQ1 | FAM, BHQ-1 |
| RNAse P forward primer | AGA TTT GGA CCT GCG AGC G | None |
| RNAse P reverse primer | GAG CGG CTG TCT CCA CAA GT | None |
| RNAse P probe | FAM – TTC TGA CCT GAA GGC TCT GCG CG – BHQ-1 | FAM, BHQ-1 |
1TaqMan® probes are labeled at the 5′-end with the reporter molecule 6-carboxyfluorescein (FAM) and with the quencher, Black Hole Quencher 1 (BHQ-1) (Biosearch Technologies, Inc., Novato, CA) at the 3′-end