Table 3. Statistics of aged actomyosin in the rigor state.
Refinement and model building statistics of aged F-actin-PHD in complex with myosin-V in the rigor state.
| Rigor state: aged F-actin-PHD + myosin-Va-LC | |||||
|---|---|---|---|---|---|
| Central 3er/2er | Central 1er(subtracted) | Class 1 | Class 2 | Class 4 | |
| 3D refinement statistics | |||||
| Number of helical segments | 299,784 | 299,784 | 94,077 | 102,818 | 81,757 |
| Resolution (Å) | 3.2 | 3.3 | 3.5 | 3.5 | 3.6 |
| Map sharpening factor (Å2) | –81 | –80 | –89 | –89 | –87 |
| Atomic model statistics | |||||
| Non-hydrogen atoms | 23,288 | 10,148 | 10,139 | 10,139 | 10,139 |
| Cross-correlation masked | 0.83 | 0.86 | 0.83 | 0.82 | 0.81 |
| MolProbity score | 1.28 | 1.18 | 1.24 | 1.25 | 1.31 |
| Clashscore | 5.25 | 3.97 | 4.66 | 4.81 | 5.75 |
| EMRinger score* | 3.14/3.39 | 3.41/3.10 | 2.97/3.00 | 3.53/3.00 | 3.01/3.06 |
| Bond RMSD (Å) | 0.005 | 0.014 | 0.005 | 0.005 | 0.005 |
| Angle RMSD (°) | 0.84 | 1.14 | 0.80 | 0.84 | 0.82 |
| Rotamer outliers (%) | 0.04 | 0.00 | 0.00 | 0.00 | 0.00 |
| Ramachandran favored (%) | 99.86 | 99.84 | 99.60 | 99.76 | 99.76 |
| Ramachandran outliers (%) | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
| CaBLAM outliers (%) | 0.8 | 0.9 | 0.9 | 0.9 | 0.8 |
*
Values correspond to score against the post-refined map used for real-space refinement/a map filtered to local resolution.