Table 4. Statistics of aged actomyosin in the post-rigor transition (PRT) state.
Refinement and model building statistics of aged F-actin-PHD in complex with myosin-V in the PRT state (bound to AppNHp).
|
|
Post-rigor transition state: aged F-actin-PHD + myosin-Va-LC + Mg2+-AppNHp | |||||||
|---|---|---|---|---|---|---|---|---|
| Central 3er/2er | Central 1er(subtracted) | Class 1 | Class 3 | Class 4 | Class 5 | Class 6 | Class 8 | |
| 3D refinement statistics | ||||||||
| Number of helical segments | 2,446,218 | 2,446,218 | 330,197 | 365,722 | 350,069 | 321,218 | 277,487 | 343,500 |
| Resolution (Å) | 2.9 | 2.9 | 3.4 | 3.3 | 3.4 | 3.3 | 3.4 | 3.3 |
| Map sharpening factor (Å2) | –80 | –100 | –113 | –106 | –114 | –106 | –111 | –104 |
| Atomic model statistics | ||||||||
| Non-hydrogen atoms | 23,370 | 10,189 | 10,125 | 10,189 | 10,154 | 10,189 | 10,085 | 10,189 |
| Cross-correlation masked | 0.85 | 0.84 | 0.84 | 0.86 | 0.85 | 0.85 | 0.83 | 0.84 |
| MolProbity score | 1.25 | 1.15 | 1.17 | 1.24 | 1.20 | 1.26 | 1.37 | 1.18 |
| Clashscore | 4.76 | 3.56 | 3.78 | 4.64 | 4.12 | 4.99 | 6.74 | 3.95 |
| EMRinger score* | 3.29/3.45 | 3.82/3.40 | 3.35/3.07 | 3.58/3.45 | 3.18/3.35 | 2.94/2.97 | 3.01/3.01 | 3.09/2.88 |
| Bond RMSD (Å) | 0.004 | 0.012 | 0.009 | 0.014 | 0.009 | 0.014 | 0.009 | 0.005 |
| Angle RMSD (°) | 0.78 | 1.01 | 0.96 | 1.15 | 0.97 | 1.14 | 1.08 | 0.81 |
| Rotamer outliers (%) | 0.08 | 0.09 | 0.09 | 0.09 | 0.09 | 0.09 | 0.09 | 0.09 |
| Ramachandran favored (%) | 99.86 | 99.84 | 99.84 | 99.84 | 99.84 | 99.84 | 99.84 | 99.84 |
| Ramachandran outliers (%) | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
| CaBLAM outliers (%) | 1.1 | 1.2 | 1.1 | 1.1 | 1.2 | 1.2 | 1.4 | 0.7 |
*
Values correspond to score against the post-refined map used for real-space refinement/a map filtered to local resolution.