Table 5. Summary of helical symmetry parameters.

Overview of helical symmetry parameters of aged PHD-stabilized and young JASP-stabilized actomyosin-V complexes. For a direct comparison, the parameters of aged F-actin-PHD (PDB: 6T20; Pospich et al., 2020) and young F-actin-JASP (PDB: 5OOD; Merino et al., 2018) are shown alongside. Differences in both the helical rise and twist can be readily explained by errors of the pixel size, which is not identical for all data sets. Helical parameters were estimated from the atomic model of five consecutive subunits independently fitted into the map; see Pospich et al., 2017 for details. To make results more comparable, only actin subunits were considered during fitting. Note that fitting inaccuracies can also give rise to small deviations.

	Rise (Å)	Twist (°)	Pixel size (Å)
Helical symmetry
Aged F-actin-PHD+ rigor	27.82±0.02	–167.27±0.02	1.06
Aged F-actin-PHD+ ADP	27.81±0.02	–167.32±0.02	1.06
Aged F-actin-PHD+ AppNHp	27.77±0.02	–167.32±0.02	1.10
Aged F-actin-PHD (PDB: 6T20)	27.59±0.02	–166.9±0.1	1.14
Young F-actin-JASP	27.85±0.08	–166.87±0.02	1.10
Young F-actin-JASP+ Rigor	27.72±0.01	–167.06±0.02	1.10
Young F-actin-JASP (PDB: 5OOD)	27.39	–166.41	1.09