Table 6. Statistics of young actomyosin in the rigor state.
Refinement and model building statistics of young F-actin-JASP alone and in complex with myosin-V in the rigor state.
| Young F-actin-JASP | Rigor state: young F-actin-JASP + myosin-Va-LC | |||||
|---|---|---|---|---|---|---|
| Actin only3er/2er | Central 3er/2er | Central 1er(subtracted) | Class 1 | Class 2 | Class 4 | |
| 3D refinement statistics | ||||||
| Number of helical segments | 212,660 | 414,148 | 414,148 | 110,797 | 107,022 | 107,174 |
| Resolution (Å) | 3.1 | 3.2 | 3.2 | 3.6 | 3.5 | 3.6 |
| Map sharpening factor (Å2) | –56 | –83 | –50 | –55 | –49 | –54 |
| Atomic model statistics | ||||||
| Non-hydrogen atoms | 8940 | 23,278 | 10,149 | 10,169 | 10,169 | 10,156 |
| Cross-correlation masked | 0.81 | 0.84 | 0.83 | 0.84 | 0.83 | 0.83 |
| MolProbity score | 1.27 | 1.29 | 1.15 | 1.24 | 1.26 | 1.23 |
| Clashscore | 5.11 | 5.46 | 3.62 | 4.66 | 4.91 | 4.57 |
| EMRinger score* | 3.11/3.08 | 2.92/2.66 | 3.11/2.92 | 2.89/2.96 | 2.99/3.39 | 2.88/2.55 |
| Bond RMSD (Å) | 0.004 | 0.004 | 0.009 | 0.005 | 0.003 | 0.004 |
| Angle RMSD (°) | 0.915 | 0.780 | 0.950 | 0.836 | 0.807 | 0.835 |
| Rotamer outliers (%) | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
| Ramachandran favored (%) | 100.00 | 99.86 | 99.84 | 99.84 | 99.84 | 99.84 |
| Ramachandran outliers (%) | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
| CaBLAM outliers (%) | 0.27 | 0.75 | 0.90 | 0.81 | 0.65 | 0.49 |
*
Values correspond to score against the post-refined map used for real-space refinement/a map filtered to local resolution.