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Expression of rhesus LCV sVCA in COS-7 cells. (A) Immunoblot strips with cell lysates of pSG5 rhesus LCV sVCA-transfected cells; (B) cell lysates of pSG5-transfected cells. Immunoblots containing cell lysates positive (A) and negative (B) for rhesus LCV sVCA were probed with five different LCV-immune rhesus sera (lanes 1 to 5) using a cassette Mini-protean II system (Bio-Rad).
