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. 2000 Sep;38(9):3219–3225. doi: 10.1128/jcm.38.9.3219-3225.2000

FIG. 5.

FIG. 5

RT-PCR for EBER1 expression in rhesus monkey PBMC. (A) Southern blot analysis of RT-PCR-amplified products from conventional colony animals. Lanes 1 to 16 represent 16 animals randomly selected from the conventional colony. Positive (lane 19) and negative (lanes 17 and 18) controls are shown. (B) Southern blot analysis of RT-PCR-amplified products from hand-reared colony animals. Lanes 1 to 16 represent 16 randomly selected hand-reared animals. Positive (lanes 17 and 18) and negative (lane 19) controls are shown. (C) Semiquantitation of rhesus LCV EBER1 by real-time RT-PCR. The EBER1 copy number per 80 ng of total cellular RNA is shown. The limit of detection (50 copies/80 ng of total RNA) is shown by the dotted line.