Figure 7.
Transcriptomic analysis of immune genes after vaccination with DDV
(A) C57BL/6J (n = 5/group) mice were immunized with either pVAX1 or DDV, animals were sacrificed 14 days after 2nd booster dose, and lymph nodes were harvested. Gene expression of immune genes were measured in lymph nodes. (B) Principal-component analysis (PCA) of immune gene expression following vaccination with DDV or pVAX1 control. (C) Volcano plots of fold change of DDV versus pVAX1 control (x axis) and log10 p value of DDV versus pVAX1 control (y axis). A deviation of 1.5 log2 fold change in gene expression was set as the cut-off value. (D) Differentially expressed genes DDV and plasmid control in lymph nodes presented as heatmap of Z scores. (E–M) Lymph node transcriptomic data 14 days after 2nd booster immunization showing Nanostring counts per 50 ng RNA of selected IFN and inflammatory genes. Data are representative of three independent experiments. Values are depicted are mean ± SD. p values were determined by Student’s unpaired t test; ∗p ≤ 0.05, ∗∗p ≤ 0.005. The study design schematic diagram was created with BioRender.com.