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. Author manuscript; available in PMC: 2022 Apr 15.
Published in final edited form as: Sci Immunol. 2021 Oct 15;6(64):eabh0707. doi: 10.1126/sciimmunol.abh0707

Fig. 6. Ubiquitination of MHCII and CD86 promotes TH2 cell immune development in a cooperative fashion.

Fig. 6.

(A) Percentages of IL-4–competent CD4+ T cells in medLNs of WT or MHCIIK>R mice at day 7 after HDM challenge (experimental outline shown in Fig. 3A). Untreated mice were used as negative controls. Data are pooled from four independent experiments with each experiment having five to six mice per treated group. (B) Total numbers of IL-4– or IL-13–competent CD4+ T cells in lungs of WT or MHCIIK>R mice at day 14 after repetitive HDM challenge (experimental outline shown in Fig. 1A). Untreated mice were used as negative controls. Data are pooled from three independent experiments with each experiment having five to six mice per treated group. (C) Percentages of IL-4–competent CD4+ T cells in medLNs of WT or CD86K>R mice after HDM challenge. Untreated mice were used as negative controls. Data are pooled from two independent experiments with each experiment having seven to eight mice per treated group. (D) Total numbers of IL-4– or IL-13–competent CD4+ T cells in lungs of WT or CD86K>R mice at day 14 after repetitive HDM challenge. Untreated mice were used as negative controls. Data are pooled from two independent experiments with each experiment having six to eight mice per treated group. (E) Percentages of IL-4–competent CD4+ T cells in medLNs of WT or MHCIIK>RCD86K>R mice at day 7 after HDM challenge. Untreated mice were used as negative controls. Data are pooled from two independent experiments with each experiment having eight mice per treated group. (F) Total numbers of IL-4– or IL-13–competent CD4+ T cells in lungs of WT or MHCIIK>RCD86K>R mice at day 14 after repetitive HDM challenge. Untreated mice were used as negative controls. Data are pooled from two independent experiments with each experiment having four to seven mice per treated group. Data are shown as means ± SEM. Statistical significance was determined by one-way ANOVA with Tukey’s multiple comparisons test. *P < 0.05; **P < 0.01; ***P < 0.001.