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. 2021 Dec 30;2021:1328444. doi: 10.1155/2021/1328444

Figure 2.

Figure 2

ZC3H13 alleviates cellular proliferative abilities of HCC cells. (a) RT-qPCR as well as (b, c) western blotting for detection of ZC3H13 expression in Hep3B and HUH-7 cells with siRNAs targeting ZC3H13. (d) RT-qPCR as well as (e, f) western blotting examining ZC3H13 expression for Hep3B as well as HUH-7 cells with ZC3H13 overexpression vectors. (g, h) CCK-8 examining the cellular growth for Hep3B as well as HUH-7 cells with ZC3H13 deficiency. (i, j) CCK-8 for evaluation for Hep3B as well as HUH-7 cells with ZC3H13 overexpression. (k–m) Clonogenicity assay for investigation of the colony formation for Hep3B as well as HUH-7 cells with ZC3H13 deficiency or overexpression. ∗∗∗P < 0.001; ∗∗∗∗P < 0.0001.