TABLE 2.

Sensitivity levels achieved by the 40-cycle PCR assay with PBS or urine specimens spiked with various amount of S. saprophyticus ATCC 15305 cells

Sample preparation	Detection limit
	CFU/PCR mixture	CFU/ml of PBS or urine
Spiked PBS
IDI DNA extraction kit	0.5	500
No treatment	650	650,000
Spiked urine samplesa
IDI DNA extraction kit	0.3–1.4	300–1,400
No treatment	700–800	700,000–800,000

^aThe given ranges of detection limits are for five urine samples.