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. Author manuscript; available in PMC: 2022 Jul 1.
Published in final edited form as: Mol Cancer Res. 2021 Sep 30;20(1):150–160. doi: 10.1158/1541-7786.MCR-21-0257

Figure 3. Inhibition of Hh/GLI signaling impairs cellular adaptation in hypoxic conditions.

Figure 3.

(A) Cells stably abrogated for GLI1 expression support significantly reduced HRE-luciferase reporter activity in SUM1315 and SUM159 cells, in hypoxia. (B) Classical HIF-1α target gene CAIX is upregulated in hypoxic conditions. Stable GLI1 silencing alleviates this increase. (C) In hypoxia, glucose consumption is significantly elevated. Stable GLI1 silencing abrogates elevated glucose consumption in hypoxia. (D) Elevated lactate production in hypoxia is mitigated by stable GLI1 silencing. Statistical significance was determined with a two-way ANOVA and Tukey’s multiple comparison test for each condition. Statistical significance was determined with two-way ANOVA Tukey’s multiple comparison test, only comparison between control and shGLI1 groups in normoxia and hypoxia conditions are shown. All error bars depict the SEM.