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. Author manuscript; available in PMC: 2022 Jul 1.
Published in final edited form as: Mol Cancer Res. 2021 Sep 30;20(1):150–160. doi: 10.1158/1541-7786.MCR-21-0257

Figure 5. Inhibition of Hh signaling in 4T1 syngeneic tumor mouse model.

Figure 5.

(A) Transcript levels of Vegf, Ptch1, Gli1, and Gli2 are significantly decreased in 4T1 cells treated with the GLI inhibitor, GANT61. Statistical significance was determined with one-way ANOVA Tukey’s multiple comparison test. Data depicted as fold change +/− SEM. (B) Vismodegib treatment decreased the number of Ptch1-positive tumor cells relative to tumors from mice administered vehicle control. Ptch1 was visualized by immunofluorescence. The graph represents the percentage of quantified Ptch1 positive tumor cells per total number of tumor cells per field. (C) 4T1 cells inhibited for Hh signaling with GANT61 and Vismodegib demonstrate a marked decrease in HIF-1α accumulation in hypoxic condition. (D) 4T1 shGLI1 cells show decreased accumulation of HIF-1α in hypoxic conditions compared to NT control cells.