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. Author manuscript; available in PMC: 2022 Jul 1.
Published in final edited form as: Clin Cancer Res. 2021 Aug 23;28(1):187–200. doi: 10.1158/1078-0432.CCR-21-2092

Figure 2. Metabolic profiles of mSDH GIST models recapitulate succinate dehydrogenase deficiency.

Figure 2.

A. Per cell abundance of succinate relative to GIST882 in mSDH GIST models grown for 48 h (n = 3). One-way ANOVA (multiple comparisons) was performed for statistical analysis with ****, P<0.0001.

B. Ratio of intracellular succinate-to-fumarate concentrations relative to GIST882 in mSDH GIST models grown for 48 h (n = 3). One-way ANOVA (multiple comparisons) was performed for statistical analysis with P>0.05 (ns), ****, P<0.0001.

C. Oxygen consumption rate (OCR) trace of intact mSDH GIST models (n = 5).

D. Basal respiration rate of intact mSDH GIST models (n = 5). One-way ANOVA (multiple comparisons) was performed for statistical analysis with ****, P<0.0001.

E. Maximal uncoupled respiration driven by succinate relative to WT in permeabilized mSDH GIST models (n = 5). One-way ANOVA was performed. ***, P<0.001; ****, P<0.0001. Maximal uncoupled respiration driven by NADH relative to WT in permeabilized mSDH GIST models (n = 5). One-way ANOVA was performed. ***, P<0.001; ****, P<0.0001.

F. Ratio of intracellular α-ketoglutarate (α-KG) to citrate relative to GIST882 in mSDH GIST models grown for 48 h (n = 3).

G. Atom transition diagram of reductive glutamine catabolism using a [U-13C5] glutamine tracer. Open circles represent 12C, closed circles represent 13C carbon atoms.

H. Percent labeling of M5 citrate from [U-13C5]-glutamine in mSDH GIST models grown for 48 h (n = 3). Data are represented as mean ± SEM with biological replicates as indicated. One-way ANOVA was performed for statistical analysis with P>0.05 (ns), *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001.