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A summary of the experimental conditions is shown.
| Cell substrate | Ultrasound | Media | Culture | Exogenous TGF-β1 | SB431542 |
|---|---|---|---|---|---|
| PDMS + TCP | None | Complete | 5 d | +/− | - |
| Fibrin | None | Complete | 5 d | +/− | - |
| Fibrin | In situ | Complete | 5 d | +/− | - |
| ARS | None | Complete | 5 d | +/− | - |
| ARS | Ex situ | Complete | 5 d | +/− | - |
| ARS | In situ | Complete | 5 d | +/− | - |
| PDMS + TCP | None | Starvation | 3 d | - | +/− |
| ARS | None | Starvation | 3 d | - | +/− |
| ARS | Ex situ | Starvation | 3 d | - | +/− |
| ARS | In situ | Starvation | 3 d | - | +/− |
