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. 2021 Oct 11;20(1):114–126. doi: 10.1158/1541-7786.MCR-21-0277

Figure 5.

Figure 5. Effects of overexpression of p110α on D556 and D283 cell survival. A, p110α expression levels in transfected D556 and 293T cells. The effects of increased p110α expression on D556 cell viability determined by trypan blue (B) and and Annexin V/PI (C) staining. D, The effects of increased p110α expression on cell viability in p53-low D283 cells. E, Suppression of p110α-enhanced chemosensitivity by RAD001 (1 μmol/L). F, RPPA analysis performed on p110α transfected D556 cells and treated with VMY (30 μmol/L) or doxorubicin (DOX, 4.5μmol/L). A panel of 159 total and phosphoproteins was used. Data are represented as fold change in protein levels in p110α versus vector control cells. Key medulloblastoma-related proteins are shown in red. Data are mean ± SD of three independent experiments. P: *, <0.05; **, <0.01; ***, <0.001.

Effects of overexpression of p110α on D556 and D283 cell survival. A, p110α expression levels in transfected D556 and 293T cells. The effects of increased p110α expression on D556 cell viability determined by trypan blue (B) and and Annexin V/PI (C) staining. D, The effects of increased p110α expression on cell viability in p53-low D283 cells. E, Suppression of p110α-enhanced chemosensitivity by RAD001 (1 μmol/L). F, RPPA analysis performed on p110α transfected D556 cells and treated with VMY (30 μmol/L) or doxorubicin (DOX, 4.5μmol/L). A panel of 159 total and phosphoproteins was used. Data are represented as fold change in protein levels in p110α versus vector control cells. Key medulloblastoma-related proteins are shown in red. Data are mean ± SD of three independent experiments. P: *, <0.05; **, <0.01; ***, <0.001.