Skip to main content
. 2021 Dec 24;15:793161. doi: 10.3389/fnana.2021.793161

FIGURE 4.

FIGURE 4

dA4 axonal projections. (A) A schematic sagittal section of the climbing fiber (CF) circuit of the dA4 subclass. dA4-derived ION (purple circle) is shown to project axonal trajectory (green line) to the Purkinje cell layer of the cerebellum (green circles). (B,C) Sagital sections from E9.5 (B) or E13.5 (C) chick embryos that were electroporated at E2.5 with a Ptf1a enhancer:Cre based plasmid together with lox-membranal GFP-lox plasmid (ptf1a:mGFP) to specifically label dA4 neurons at r7. The section in (B) shows dA4-derived axonal trajectories ascending from the caudal hindbrain toward the developing cerebellum. The section in (C) shows dA4-derived axonal trajectories that terminate in the cerebellum. An outer granular layer of the cerebellum is marked by Axonin 1. Bars, 50 μm. ION, inferior olivary nuclei; Ax-1, axonin 1.