Fig. 4.

(A–D) Impact of EGFR- or AT1R activation on EGFR, phosphoEGFR, phosphoERK1/2, cFOS expression in HEK cells. (A, B) Results from immunoblot analysis of protein expression. (C) cFOS-mRNA expression determined by qRT-PCR. (D) SRF activity, determined by reporter gene assay and cFOS protein expression, determined by immunofluorescence. (E) Immunoblot analysis of c-JUN, SRF, myocardin, MRTF-A or ELK-1 expression showed no effect of EGF, AII or AII + EGF. Representative blots of N = 3. (F, G) Impact of EGFR transactivation by AT1R activation via HB-EGF on EGFR, phosphoEGFR, phosphoERK1/2 and cFOS protein expression in HEK cells. *p < 0.05. N for (B, C, G) = 4. N for D = 12