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. 2021 Jul 31;17(1):211–218. doi: 10.1007/s11548-021-02458-2

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Visualization of the size-exclusion column measurements for ICG-SPION. Fluorescence intensity is shown of the first fraction after purification of a column with ICG and a column with ICG-SPION. This clearly shows a rise of fluorescence emission at 810 nm for ICG-SPION, indicating that at least part of the ICG has bound non-covalently to the SPION particles. The intensity values were corrected by subtracting the SPION background intensity and normalized to that of ICG-SPION