Fig. 5. Mice harboring a conditional deletion of Bcl2 potentiates apoptosis of monocyte-derived macrophages.

IPF lung macrophages were transfected with human scrambled or Bcl2 siRNA. A Immunoblot for Bcl-2 and B caspase-3 activity, n = 10. C Lung macrophages from bleomycin- or saline-exposed Bcl2^−/−Csf1r^MeriCreMer mice and their Bcl2^fl/fl littermates were stained with TUNEL and imaged by confocal microscopy. Scale bars, 20 μm. Monocyte-derived macrophages (MDM) from bleomycin- or saline-exposed Bcl2^−/−Csf1r^MeriCreMer mice and their Bcl2^fl/fl littermates were D stained with TUNEL and imaged by confocal microscopy was quantified, n = 3, or E stained for the detection of Annexin V by flow cytometry, n = 4. Bcl2^−/−Csf1r^MeriCreMer mice were exposed to Bleomycin or saline. Mice were intraperitoneally injected daily with corn oil dissolved tamoxifen or corn oil from day 12 until day 20 (20 mg/kg; break per every two injections), and mice were lavaged at day 28. F BAL macrophages were prepared for detection of caspase-3 activities, n = 3, and Bcl-2 by immunoblot analysis. One-way ANOVA with Tukey’s post hoc comparison. Two-tailed student’s t-test for B. *p ≤ 0.05, **p ≤ 0.01, and ***p ≤ 0.001. See also Fig. S5.