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. 2021 Jul 14;29(1):28–39. doi: 10.1038/s41418-021-00832-w

Fig. 3. The catalytic activity of Drice is required for Imd pathway regulation in the intestine.

Fig. 3

A Relative Drosocin and Diptericin mRNA levels analysed with qPCR in adult CantonS, Diap27c, UbiGal4;UAS-Drice-RNAi, UAS-DriceWT/UbiGal4;UAS-Drice-RNAi and UAS-DriceC211A/UbiGal4;UAS-Drice-RNAi flies, n = 7. B Adult female intestines from NP1-Gal4 and NP1-Gal4;UAS-p35 flies were dissected and lysed, and the caspase-3/7 activity was assessed after addition of Apo-ONE reagent by measuring fluorescence at 499/521 nm, n = 3. C Relative Drosocin and Diptericin mRNA levels analysed with qPCR in adult NP1-Gal4, UAS-p35, Diap27c and NP1-Gal4;UAS-p35 flies, n = 9. D Adult female intestines from DaGal4, Diptericin-LacZ and NP1-Gal4;UAS-p35/Diptericin-LacZ flies were dissected and stained for β-galactosidase activity, n = 3. Data represent mean ± SEM, *p < 0.05.