Fig. 5. Drice does not restrain pathogen-induced inflammatory signalling.

Relative Drosocin and Diptericin mRNA levels analysed with qPCR in Canton^S, Diap2^7c, Drice¹⁷ (A) UbiGal4;UAS-Drice-RNAi (B) and in DaGal4, Diap2^7c and UAS-Drice^WT;DaGal4 (C) flies 5 h after septic infection with the gram-negative bacteria Ecc15, n ≥ 3. Adult Canton^S, Diap2^7c and Drice¹⁷ (D) or DaGal4, Diap2^7c and UAS-Drice^WT;DaGal4 (E) flies were subjected to septic injury with Ecc15 and their survival was monitored over time, n = 3. F DaGal4, UbiGal4, Diap2^7c, UAS-Drice^WT;DaGal4 and UbiGal4 > Drice-RNAi flies were infected by feeding with E. coli for 24 h and the bacterial load was assessed by counting colony-forming units (CFU), n = 4. G The induction of K63-Ub chains was analysed in Canton^S, UAS-Drice^WT;DaGal4 and UbiGal4 > Drice-RNAi flies 5 h after septic infection with Ecc15. Ubiquitin chains were isolated with GST-TUBE under denaturing conditions and samples analysed by western blotting with α-K63, α-Diap2, α-Drice and α-Actin antibodies, n = 3. The relative protein level of K63-Ub chains was quantified. H Adult flies were infected by feeding Ecc15 16 h, their guts were dissected, lysed and used in western blot analysis with α-Drice, α-Diap2 and α-Actin antibodies, n = 4. The relative protein levels of full-length Diap2, cleaved Diap2 and Drice were quantified. Data represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.