Table 2.
Nucleotide sequences of specific polymerase chain reaction primers
| Gene1) | Primer sequence | Annealing Temp (°C) | Length (bp) | Gen Bank ID no. |
|---|---|---|---|---|
| SGLT1 | F-TGTCTCTCTGGCAAGAACATGTC R-GGGCAAGAGCTTCAGGTATCC |
60 | 71 | XM_415247 |
| GLUT5 | F-TTGCTGGCTTTGGGTTGTG R-GGAGGTTGAGGGCCAAAGTC |
60 | 60 | XM_417596 |
| PepT1 | F-CCCCTGAGGAGGATCACTT R-CAAAAGAGCAGCAGCAACGA |
60 | 66 | NM_204365 |
| GAPDH | F-GCCGTCCTCTCTGGCAAAG R-TGTAAACCATGTAGTTCAGATCGA |
60 | 73 | MN_204305 |
1)
SGLT1, Na+-D-glucose co-transporter 1; GLUT5, Fructose transporter; Pep T1, H+-dependent oligopeptide transporter; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.