Figure 1.
Decreased METTL14 expression correlates to poor prognosis in ccRCC patients.
(A) The m6A levels of the total extracted RNA from nine pairs of ccRCC tissues. (B) The qRT-PCR assay results showing mRNA levels of METTL3, METTL14, WTAP, FTO, and ALKBH5 compared with GAPDH controls in 50 pairs of ccRCC tissues. (C) The protein levels of METTL14 in 32 pairs of ccRCC tissues. N, normal tissue; T, tumor tissue. (D) Representative IHC images of METTL14 in 40 ccRCC tissues from the SRRSH cohort. The IHC scores of each sample were calculated. Dots, IHC score; lines, pairs of normal and tumor tissues. Scale bars represent 10 μm. SRRSH, Sir Run Run Shaw Hospital. (E) Representative IHC images of METTL14 in tissue microarrays obtained from 79 pairs of ccRCC tissues and their corresponding IHC scores. Dots, IHC score; lines, pairs of normal and tumor tissues. Scale bars represent 200 μm. (F) Kaplan-Meier survival curves showing overall survival of 79 ccRCC patients based on the IHC score. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001; ns, not significant. Data are representative of three independent experiments.