Figure 3.

Lnc-LSG1 was identified as a downstream target of METTL14

(A) MeRIP-seq identified four lncRNAs whose m⁶A levels were significantly decreased following METTL14 knockdown in 786-O and Caki-1 cells. (B) The RIP and qRT-PCR assays showed that m⁶A could modify Lnc-ZNF121 and Lnc-LSG1 m⁶A levels. In addition, knockdown of METTL14 could decrease their m⁶A levels. (C) Integrative Genomics Viewer plots showing a decreased m⁶A peak at the Lnc-LSG1 transcripts. (D) Transwell assay showed that Lnc-LSG1 could regulate the migration and invasion of the 786-O cells. (E) Inhibition of Lnc-LSG1 suppressed the migratory and invasive abilities of 786-O cells increased by shMETTL14-1. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001; ns, not significant. Data are presented as the mean ± SD of at least three independent experiments.