Figure 7.

Validation of RBM15 expression in OS cell lines and in human clinical specimens

(A) Quantitative real-time PCR analysis of RBM15 expression in the osteosarcoma cell lines. (B) Western blot analysis of RBM15 expression in osteosarcoma cell lines. GAPDH was used as a loading control. (C) The representative image of RBM15 protein expression in human primary and metastatic osteosarcoma tissue by immunohistochemistry. (D) Quantitative real-time PCR validation of RBM15 overexpression in the HOS cell line. (E) Western blot validation of RBM15 overexpression in the HOS cell line. GAPDH was used as a loading control. (F) Quantitative real-time PCR validation of RBM15 knockdown in the MNNG cell line. (G) Western blot validation of RBM15 knockdown in the MNNG cell line. GAPDH was used as a loading control. All bar plot data have been presented as mean ± standard deviation of three independent experiments. ∗∗p < 0.01, ∗∗∗p < 0.001.