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. 2021 Dec 17;27:562–576. doi: 10.1016/j.omtn.2021.12.026

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© 2021 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Preparation and characterization of AuNCs and AuNC-siRNA

(A) TEM images of AuNCs. (B) The mean diameter of AuNCs (n = 10 per group). (C) TEM images of AuNC-siRNA. (D) Mean diameter of AuNC-siRNA (n = 10 per group). (E) Surface charge of IKKβ siRNA, AuNCs, and AuNC-siRNA (n = 3 per group). (F) XPS for the C, N, O, S, P, and Au on AuNC and AuNC-siRNA surface. (G) Live/dead cell staining for TSPCs treated with different concentrations of AuNCs. Green fluorescence indicates live cells and red fluorescence indicates dead cells. (H) Quantification of TSPCs viability (n = 3 per group). (I) A Cell Counting Kit-8 assay for the effects of different AuNC concentrations on TSPCs proliferation (n = 3 per group). (J) The effects of different AuNCs concentrations on cell cycle distribution (n = 3 per group).