Fig. 1.

Generation of recombinant MCMV vectors. A, B Schematic images of the recombinant MCMV vector genome. A The HA gene of IAV PR8 was inserted along with minimal hMIEP in the m157 locus. B The SARS-CoV-2 spike ORF was inserted in place of ie2. Western blotting of purified virus stocks of MCMV^S, MCMV^HA and MCMV^WT was performed with antibodies against (C) IAV HA or (D) SARS CoV-2 S. As controls, we used (C) ie1 or (D) MCK-2 proteins of MCMV. E M210B4 cells were infected with MCMV^S and analyzed at 2 days post-infection (dpi) by immunofluorescence staining of the spike protein to detect its expression or of a GFP protein expressed by the ie1/3 promoter to identify MCMV-infected cells. DAPI staining was used to identify nucleated cells