Fig. 3.

eNOS is important for VEGF-induced endothelial cell sprouting. A Representative images from spheroid-based angiogenesis assays generated from BAECs transfected with control siRNA (CT-siRNA), eNOS-siRNA, Par3-siRNA or both (Par3 + eNOS-siRNA) and exposed to VEGF (40 ng/mL) or left untreated. Pictures were taken 24 h after embedding in a collagen matrix. B Quantification of the number of capillary-like sprouts from spheroids was measured following VEGF stimulation. C The length of sprouts was measured and expressed relatively to spheroids that were not stimulated with VEGF. D The number of extensions per tip-cell was quantified. Data are represented as mean ± SEM of three experiments with at least 8 spheroids per condition per experiment. *p < 0.05