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. 2021 Dec 31;79(1):37. doi: 10.1007/s00018-021-04042-y

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — eNOS is an essential mediator of retinal angiogenesis. A High-resolution imaging of Isolectin B4–stained mouse retina at postnatal day 5 (P5) and P11 from eNOS^+/+ and eNOS^−/− mice. The percentage of the vascular area and vessel density was measured in B P5 (n = 7 per group) and C P11 (n = 9 per group) retinas from eNOS^+/+ and eNOS^−/− mice. D Representative images from P5 retinas showing tip-cells and filopodia. The numbers of E tip-cells, F filopodia per tip-cell, and G the percentage of oriented cells (cells pointing in between 60° and 120° perpendicular to the vascular front) were determined in retinas from eNOS^+/+ and eNOS^−/− mice (n = 10 retinas per group). White dashed lines in D indicates the orientation of the vascular front. Results are displayed as mean values ± SEM. *p < 0.05