Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Dec 31;79(1):37. doi: 10.1007/s00018-021-04042-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 7 — Single-cell RNA-sequencing analysis of ECs of retinas from eNOS^−/− mice compared to WT at P14 of OIR. A Uniform Manifold Approximation and Projection (UMAP) analysis and representation of single-cell gene expression of P14 OIR retinas from eNOS^+/+ and eNOS^−/− (n = 4 per group) shows three identified EC subpopulations (tip, structural and proliferative). B Bar graph of the Normalized Enrichment Score (NES) of the ¨GO: 0032878 Regulation of establishment or maintenance of cell polarity¨ in tip, structural and proliferative ECs. C Gene set enrichment analysis (GSEA) of curated cell polarity gene signature in P14 OIR eNOS^-/- and eNOS^+/+ retina in tip, structural and proliferative ECs (p-value and NES of each GSEA are indicated). D Heatmaps of the ¨GO: 0045177 Apical Part of Cell¨ showing enrichment in deficient eNOS P14 OIR retinas in the three sub-populations of ECs. E Dot plot demonstrates enrichment of polarity genes expression in the three EC subpopulations eNOS^−/− compared to eNOS^+/+. Dot size corresponds to proportion of cells within the group expressing each transcript, and dot color corresponds to expression level (green high and red low expression)